goat polyclonal anti vegf 164  (R&D Systems)

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Chronic hypoxia exacerbates diabetic glomerulosclerosis through mesangiolysis and podocyte injury in db/db mice.

doi: 10.1093/ndt/gfaa074

Figure Lengend Snippet: FIGURE 2: Urinalysis of the effects of hypoxia in db/db mice. (A–C) Comparison of (A) urinary albumin (n ¼ 6–8), (B) urinary PCX (n ¼ 6–9) and (C) urinary VEGF-A (n ¼ 6–8) levels between hypoxic (filled square) and age-matched normoxic (open square) mice. Vertical columns and bars depict mean 6 SD. (D and E) Photomicrographs of IF staining of urinary PCX in samples from db/db mice collected af- ter 1 week of hypoxia. (D) Negative PCX staining in sediments obtained with conventional centrifugation. (E) Strong granular PCX stain- ing in the bottom fraction after the subsequent UC of the supernatants obtained with conventional centrifugation. Bars ¼ 20 lm. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Similarly, goat anti-mouse VEGF polyclonal antibody (AF-493-NA; R&D Systems), rabbit anti-transforming growth factor-b1 (TGF-b1) monoclonal antibody (ab215715; Abcam) and mouse anti-eNOS monoclonal antibody (ab76198; Abcam) were used.

Techniques: Comparison, Staining, Centrifugation

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Chronic hypoxia exacerbates diabetic glomerulosclerosis through mesangiolysis and podocyte injury in db/db mice.

doi: 10.1093/ndt/gfaa074

Figure Lengend Snippet: FIGURE 4: Representative glomerular immunohistological stainings of (A) CD34-positive endothelial cells, (C) WT-1-positive podocytes, (E) VEGF-positive cells, (G) F4/80-positive macrophages (indicated by arrow), (I) TGF-b1and (K) eNOS (K) in 12- and 24-week-old normoxic and hypoxic db/db mice. The graphs on the right show the comparison of positive area densities (%) of (B) CD34, (D) WT-1, (F) VEGF, (J) TGF-b1and (L) eNOS and (H) the average number of F4/80-positive macrophages per glomerulus among normoxic (open circle) and hypoxic (filled circle) 12-week-old mice and normoxic (open triangle) and hypoxic (filled triangle) 24-week-old mice. Each symbol represents an individual. Horizontal lines and boxes are the same as those in Figure 3. *P < 0.05, **P < 0.01, ***P < 0.005 and ****P < 0.001.

Article Snippet: Similarly, goat anti-mouse VEGF polyclonal antibody (AF-493-NA; R&D Systems), rabbit anti-transforming growth factor-b1 (TGF-b1) monoclonal antibody (ab215715; Abcam) and mouse anti-eNOS monoclonal antibody (ab76198; Abcam) were used.

Techniques: Comparison

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Chronic hypoxia exacerbates diabetic glomerulosclerosis through mesangiolysis and podocyte injury in db/db mice.

doi: 10.1093/ndt/gfaa074

Figure Lengend Snippet: FIGURE 5: Comparison of relative mRNA levels within LCM-iso- lated glomeruli encoding (A) VEGF-A, (B) VEGFR-2, (C) eNOS, (D) TGF-b1, (E) MCP-1and (F) PAI-1 between 24-week-old nor- moxic (open triangle) and hypoxic (filled triangle) db/db mice. Each symbol represents an individual. Horizontal lines and boxes are the same as those in Figure 3. *P < 0.05 and **P < 0.01.

Article Snippet: Similarly, goat anti-mouse VEGF polyclonal antibody (AF-493-NA; R&D Systems), rabbit anti-transforming growth factor-b1 (TGF-b1) monoclonal antibody (ab215715; Abcam) and mouse anti-eNOS monoclonal antibody (ab76198; Abcam) were used.

Techniques: Comparison

Journal: Journal of pharmacological sciences

Article Title: Lymphangiogenesis induced by vascular endothelial growth factor receptor 1 signaling contributes to the progression of endometriosis in mice.

doi: 10.1016/j.jphs.2020.05.003

Figure Lengend Snippet: Fig. 4. The mRNA expression levels of Vegfc and Vegfd in cultured macrophages and fibroblasts. (A) The effects of PlGF (0 and 10 ng/ml) on the levels of the mRNAs encoding VEGFR1, VEGF-C, and VEGF-D in cultured bone marrow-derived macrophages from WT and TK-/- mice. Data are expressed as the mean ± SEM (n ¼ 4e6 mice per group). *P < 0.05. (B) The effects of PlGF (0 and 1 ng/ml) on the levels of the mRNAs encoding VEGFR1, VEGF-C, and VEGF-D in L929 cells in the presence of an anti-VEGFR1 antibody (10 ng/ml) or IgG. Data are expressed as the mean ± SEM (n ¼ 4e6 mice per group). *P < 0.05.

Article Snippet: The sections were incubated with the following primary antibodies at 4 C overnight: a rabbit anti-mouse VEGFR1 polyclonal antibody (1:200; Abcam), a goat anti-mouse VEGFR1 polyclonal antibody (1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA), a rabbit anti-LYVE-1 polyclonal antibody (1:200; Abcam), a goat anti-LYVE-1 polyclonal antibody (1:200; R&D Systems Inc., Minneapolis, MN, USA), a rabbit anti-VEGF-C polyclonal antibody (1:200; Abcam), a goat anti-VEGFD polyclonal antibody (1:40; R&D Systems Inc.), a rat anti-mouse CD11b monoclonal antibody (1:200; BD Biosciences, San Jose, CA, USA), a rabbit anti-mouse S100A4 polyclonal antibody (1:200; Abcam), or a goat anti-mouse S100A4 polyclonal antibody (1:100; OriGene Technologies, Rockville, MD, USA).

Techniques: Expressing, Cell Culture, Derivative Assay